Author(s): Thomi R, Yerly D, Yawalkar N, Simon D, Schlapbach C, Hunger RE
BACKGROUND: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. Its immunopathogenic mechanisms are still poorly understood. Previous studies could demonstrate that the pro-inflammatory cytokine Interleukin-32 (IL-32) is implicated in the pathogenesis of other inflammatory diseases.
OBJECTIVES: The aim of our study was to investigate the tissue expression and systemic levels of IL-32 as well as its cellular sources in HS patients in comparison to healthy donors and to patients suffering from two other inflammatory skin diseases: psoriasis (PS) and atopic dermatitis (AD).
METHODS: Tissue samples were obtained from healthy skin and lesional HS, PS and AD skin to analyse the expression of IL-32 by immunohistochemistry and semi-quantitative real-time PCR. The cellular source of the cytokine was determined by double immunofluorescence staining. Serum of the four donors groups was used to measure systemic levels of IL-32 by enzyme-linked immunosorbent assay (ELISA).
RESULTS: IL-32 was upregulated in HS patients in both lesional skin and serum when compared to healthy donors or AD and PS patients. In HS, IL-32 was found to be expressed by NK cells, T cells, macrophages and dendritic cells in highly infiltrated areas of the dermis. High IL-32 mRNA levels in lesional HS skin coincided with high amounts of T cells and macrophages present. Additionally, IL-32 mRNA levels in lesional HS skin correlate positively with IFNγ and IL-17 and negatively with IL-13.
CONCLUSIONS: Our findings suggest that IL-32 is overexpressed in HS. Targeting IL-32 may therefore represent a new therapeutic option for the treatment of this recalcitrant disease. This article is protected by copyright. All rights reserved.
Dermatology Journal and/or Publisher
Journal Name: The British journal of dermatology
Journal Abbreviation: Br. J. Dermatol.
Journal Date Published: 2017-03-16
National Center for Biotechnology Information
Article Source: http://www.ncbi.nlm.nih.gov/pubmed/28301691
Lasted Revision: 2017-03-16
Abstract Source: National Center for Biotechnology Information, U.S. National Library of Medicine Abstract Query for Hidradenitis suppurativa (HS).